4PZU
Crystal structure of a putative uncharacterize protein Rv3404c and likely sugar N-formyltransferase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.080, 136.450, 99.970 |
| Unit cell angles | 90.00, 103.74, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nv1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 | |
| High resolution limit [Å] | 2.100 | 9.620 | 2.150 |
| Rmerge | 0.060 | 0.026 | 0.462 |
| Number of reflections | 128170 | 1419 | 9325 |
| <I/σ(I)> | 15.22 | 38.16 | 2.97 |
| Completeness [%] | 99.8 | 96.5 | 99.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | MytuD.17389.a.A1.PW30616 at 19 mg/mL against JCSG+ screen condition D6, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 20% PEG 8000 supplemented with 25% ethylene glycol, crystal tracking ID 220349d6, unique puck ID bdu3-7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
