4PZ4
High-resolution crystal structure of the human CD44 hyaluronan binding domain in new space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.271, 70.271, 286.458 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.860 - 1.600 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 286.458 | 1.604 |
| High resolution limit [Å] | 1.598 | 1.598 |
| Rmerge | 0.063 | 0.490 |
| Number of reflections | 56356 | |
| <I/σ(I)> | 26.4 | 5.3 |
| Completeness [%] | 99.3 | 80.4 |
| Redundancy | 12.8 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 277 | 25% PEG 3350, 100 mM NACl, 100 mM HEPES, (5% DMSO), pH 7.0, hanging drop, temperature 277K |
