4PZ3
High-resolution crystal structure of the human CD44 hyaluronan binding domain complex with undefined peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.498, 60.288, 75.134 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 44.670 - 1.083 |
| R-factor | 0.1668 |
| Rwork | 0.166 |
| R-free | 0.17740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.511 |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.676 | 1.086 |
| High resolution limit [Å] | 1.083 | 1.083 |
| Rmerge | 0.031 | 0.154 |
| Number of reflections | 122395 | |
| <I/σ(I)> | 21.2 | 5.4 |
| Completeness [%] | 97.1 | 69.3 |
| Redundancy | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 277 | 30% PEG MME 5000, 100 mM MES, 200 mM (NH4)2SO4, 5%DMSO, pH 6.5, hanging drop, temperature 277K |
