4PY8
Crystal structure of Fab 3.1 in complex with the 1918 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97549 |
| Spacegroup name | H 3 |
| Unit cell lengths | 135.060, 135.060, 230.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.841 - 2.910 |
| R-factor | 0.19551 |
| Rwork | 0.193 |
| R-free | 0.24186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.841 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 34347 | |
| Completeness [%] | 99.6 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 15% PEG3350, 0.1 M magnesium sulfate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
