4PXW
Crystal structure of human DCAF1 WD40 repeats (Q1250L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.888, 99.422, 84.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.720 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cc9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.683 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.900 | 48.900 | 1.750 |
| High resolution limit [Å] | 1.720 | 9.100 | 1.720 |
| Rmerge | 0.131 | 0.012 | 0.012 |
| Total number of observations | 3315 | 26202 | |
| Number of reflections | 67989 | ||
| <I/σ(I)> | 11.4 | 33 | 1.9 |
| Completeness [%] | 100.0 | 99 | 99.8 |
| Redundancy | 7.3 | 6.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.1 M sodium hepes, 0.2 M lithium sulfate, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, temperature 291K |
