4PXF
Crystal structure of the active G-protein-coupled receptor opsin in complex with the finger-loop peptide derived from the full-length arrestin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.622, 242.622, 110.216 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.040 - 2.750 |
| R-factor | 0.21659 |
| Rwork | 0.215 |
| R-free | 0.25075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dqb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.040 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.050 | 0.944 |
| Number of reflections | 32166 | |
| <I/σ(I)> | 23.1 | 2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 3.2 M (NH4)2SO4 in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
