4PWX

Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	166.341, 134.501, 84.060
Unit cell angles	90.00, 113.37, 90.00

Refinement procedure

Resolution	30.000 - 5.400
Rwork	0.270
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2WOJ AND 3LKU
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (v6.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	6.000
High resolution limit [Å]	5.400	5.400
Rmerge	0.050	0.460
Number of reflections	5529
<I/σ(I)>		2
Completeness [%]	93.8	95.9
Redundancy		2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	18% PEG 3350, 0.2M potassium thiocyanate, 0.1M bis-tris propane, 5% DMSO, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K