4PT4

Crystal structure Analysis of N terminal region containing the dimerization domain and DNA binding domain of HU protein(Histone like protein-DNA binding) from Mycobacterium tuberculosis [H37Ra]

Replaces:  3C4I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	36.774, 53.974, 41.732
Unit cell angles	90.00, 97.01, 90.00

Refinement procedure

Resolution	41.420 - 2.040
R-factor	0.1906
Rwork	0.188
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p71
RMSD bond length	0.011
RMSD bond angle	1.477
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.040	4.420	2.040
Rmerge	0.092	0.061	0.391
Number of reflections	10304
<I/σ(I)>	7.5
Completeness [%]	98.7	99.8	92.9
Redundancy	3.8	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	3M Sodium formate, 0.1M Tris-Cl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K