4PQW
Crystal Structure of Phospholipase C beta 3 in Complex with PDZ1 of NHERF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1272 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.660, 50.660, 66.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.545 - 1.470 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jl7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.041 | 0.575 |
| Number of reflections | 17247 | |
| <I/σ(I)> | 21.2 | 2.7 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM sodium acetate, pH 4.6, 2.5 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
