4PPI
Crystal structure of Bcl-xL hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 153.630, 153.630, 153.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.158 - 2.851 |
R-factor | 0.2396 |
Rwork | 0.236 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b48 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.982 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.350 | 3.010 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.086 | 0.702 |
Number of reflections | 7536 | |
<I/σ(I)> | 28.3 | 5.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 38.8 | 41.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 1.0M sodium citrate, 0.1M CHES pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |