4PMW
Structure of mouse Dis3L2 in complex with oligoU RNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.927, 96.075, 157.311 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 63.182 - 2.950 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.957 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.100 | 96.070 | 3.300 |
| High resolution limit [Å] | 2.950 | 6.600 | 2.950 |
| Rmerge | 0.126 | 0.063 | 0.463 |
| Rpim | 0.080 | 0.040 | 0.290 |
| Total number of observations | 136961 | 12265 | 39093 |
| Number of reflections | 39795 | ||
| <I/σ(I)> | 8 | 16.3 | 2.9 |
| Completeness [%] | 99.9 | 99.6 | 99.9 |
| Redundancy | 3.4 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 100 mM ammonium citrate pH 5.5, 200 mM ammonium chloride, 22 % PEG 3350 |
