4PKY

ARNT/HIF transcription factor/coactivator complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97951
Spacegroup name	P 41 21 2
Unit cell lengths	96.136, 96.136, 182.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.068 - 3.200
R-factor	0.2332
Rwork	0.231
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f1o 4lpz
RMSD bond length	0.008
RMSD bond angle	0.926
Data reduction software	HKL-2000 (705a)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.070	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.148	0.077	0.629
Total number of observations	106957
Number of reflections	14853
<I/σ(I)>	6.1		1.3
Completeness [%]	99.9	99.9	98.7
Redundancy	7.2	6.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.6 M magnesium sulfate, 0.1 M MES pH 6.5, 20% (w/v) ethylene glycol