4PKH
Complex of ADP-actin With the N-terminal Actin-Binding Domain of Tropomodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.222, 135.136, 140.549 |
Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
Resolution | 44.900 - 2.150 |
R-factor | 0.2749 |
Rwork | 0.274 |
R-free | 0.32620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eqy |
RMSD bond length | 0.018 |
RMSD bond angle | 1.729 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.077 | 0.460 |
Number of reflections | 138122 | |
<I/σ(I)> | 17.03 | 2 |
Completeness [%] | 98.7 | 99.4 |
Redundancy | 5.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400 |