4PJ6
Crystal Structure of Human Insulin Regulated Aminopeptidase with Lysine in Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.031, 256.621, 71.310 |
| Unit cell angles | 90.00, 114.35, 90.00 |
Refinement procedure
| Resolution | 48.320 - 2.960 |
| R-factor | 0.18899 |
| Rwork | 0.185 |
| R-free | 0.25669 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.580 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 3.060 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Number of reflections | 46240 | |
| <I/σ(I)> | 12.4 | 1.7 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 3000, tris hydrochloride |
