4PHH

Crystal structure of Ypt7 covalently modified with GNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92045
Spacegroup name	P 1 21 1
Unit cell lengths	60.570, 72.230, 82.340
Unit cell angles	90.00, 91.49, 90.00

Refinement procedure

Resolution	48.180 - 2.350
R-factor	0.20331
Rwork	0.200
R-free	0.26301
RMSD bond length	0.015
RMSD bond angle	1.672
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	2.450
High resolution limit [Å]	2.350	2.350
Number of reflections	29719
<I/σ(I)>	18.28
Completeness [%]	99.9
Redundancy	6.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Tris pH 7, 25 % PEG 6000, 1 M LiCl