4PDK

FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae, in Complex with oleoyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	77
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	116.920, 88.690, 62.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 2.800
R-factor	0.2628
Rwork	0.259
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.148
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER ((phenix.molecular replacement: 1.8.4_1496))
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.730
High resolution limit [Å]	2.800
Number of reflections	16577
<I/σ(I)>	11.48
Completeness [%]	99.3
Redundancy	3.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	17% PEG 3350, 0.23M magnesium formate