4PCU
Crystal structure of delta516-525 E201S human cystathionine beta-synthase with AdoMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.354, 141.354, 108.528 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.805 - 3.578 |
| R-factor | 0.2582 |
| Rwork | 0.257 |
| R-free | 0.27670 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.302 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.805 |
| High resolution limit [Å] | 3.578 |
| Number of reflections | 14903 |
| <I/σ(I)> | 5.01 |
| Completeness [%] | 98.6 |
| Redundancy | 3.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (vol/vol) polyethylene glycol monomethyl ether 3350 and 0.34mM SAM |
