4PC6

Elongation factor Tu:Ts complex with bound GDPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	292
Detector technology	CCD
Collection date	2002-10-17
Detector	MAR CCD 165 mm
Wavelength(s)	1.2
Spacegroup name	P 21 21 21
Unit cell lengths	73.969, 107.467, 193.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.542 - 2.200
R-factor	0.173
Rwork	0.171
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pc1
RMSD bond length	0.004
RMSD bond angle	0.809
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.010	0.440
Number of reflections	79003
<I/σ(I)>	13.8	3.4
Completeness [%]	99.5	98.5
Redundancy	7.7	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	100mM MES, 18% PEG 6000, 5% glycerol, 152mM NH4CH3COO, 0.5mM GDPNP, 0.5mM pulvomycin, 5mM EDTA