4PAB
Crystal structure of the precursor form of rat DMGDH complexed with tetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.182, 107.215, 114.466 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 48.190 - 1.850 |
| R-factor | 0.20697 |
| Rwork | 0.206 |
| R-free | 0.23053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pj5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.288 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.190 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.392 | |
| Number of reflections | 167803 | |
| <I/σ(I)> | 22.2 | 1.5 |
| Completeness [%] | 96.7 | 90.2 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M NaSCN, 20% PEG 3350, 0.1 M Tris pH 7.5 |
