4PAA
Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.934, 130.661, 171.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.260 |
| R-factor | 0.24128 |
| Rwork | 0.241 |
| R-free | 0.26739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.168 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.297 | |
| Number of reflections | 87235 | |
| <I/σ(I)> | 11.7 | 1.9 |
| Completeness [%] | 97.1 | 90.6 |
| Redundancy | 6.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M K/Na-tartrate, 20% PEG3350 |
