4P6W

Crystal Structure of mometasone furoate-bound glucocorticoid receptor ligand binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 2 3
Unit cell lengths	130.218, 130.218, 130.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.874 - 1.951
R-factor	0.2116
Rwork	0.210
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.950
Rmerge	0.128
Number of reflections	53521
<I/σ(I)>	23.5
Completeness [%]	99.4
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1 M imidazole, pH6.5, and 1 M sodium acetate trihydrate. 30% sucrose