4P5X
Structure of the N-terminal domain of the human mitochondrial aspartate/glutamate carrier Aralar in the calcium-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.898 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 54.450, 54.450, 168.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.030 - 2.261 |
| R-factor | 0.2334 |
| Rwork | 0.231 |
| R-free | 0.27410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p5w |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.033 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.044 | 0.859 |
| Number of reflections | 14197 | |
| <I/σ(I)> | 21.3 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1M MIB, 25% PEG 1500 |
