4P5K
Structural Basis of Chronic Beryllium Disease: Bridging the Gap Between Allergy and Autoimmunity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.779, 68.738, 71.133 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 44.620 - 2.590 |
| R-factor | 0.2245 |
| Rwork | 0.221 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.626 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 26282 |
| <I/σ(I)> | 18.6 |
| Completeness [%] | 92.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 316 | 0.2 M ammonium formate and 25 % PEG 4000 in 0.1 M HEPES buffer |
