4P5B
Crystal structure of a UMP/dUMP methylase PolB from Streptomyces cacaoi bound with 5-Br dUMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9798 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.788, 59.502, 118.981 |
Unit cell angles | 90.00, 120.31, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.274 |
R-factor | 0.1666 |
Rwork | 0.164 |
R-free | 0.21554 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.102 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.274 |
Number of reflections | 46721 |
<I/σ(I)> | 11 |
Completeness [%] | 98.0 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium cacodylate, pH 6.5 and 1.4M sodium acetate |