4P4S
GMPPCP-bound stalkless-MxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.290, 66.760, 170.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.260 - 3.300 |
| R-factor | 0.23441 |
| Rwork | 0.231 |
| R-free | 0.30531 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p4u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.605 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 10950 | |
| <I/σ(I)> | 19 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 291 | 23-27% PEG 4000, 0.2 M EPPS/KOH pH 7.9, 0.5M glycerol |
