4P4R

Structural Basis of Chronic Beryllium Disease: Bridging the Gap Between Allergic Hypersensitivity and Autoimmunity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	273
Detector technology	PIXEL
Collection date	2013-07-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	77.250, 102.440, 120.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.150 - 3.000
R-factor	0.199
Rwork	0.196
R-free	0.25560
RMSD bond length	0.010
RMSD bond angle	1.300
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	90.000
High resolution limit [Å]	3.000
Number of reflections	18043
<I/σ(I)>	4.77
Completeness [%]	91.2
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NH4tartrate dibasic, 20% PEG-3350