4P2V
Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.617, 105.742, 170.402 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 49.260 - 2.510 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gkf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.371 |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1626)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.263 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.104 | 0.491 |
| Number of reflections | 92529 | |
| <I/σ(I)> | 9.32 | 2.47 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 21% PEG 400, 110 mM MgCl2, 1 mM DTT, and 100 mM Tris pH 8.0 well solution. Drop was 1ul protein (7.3mg/ml) and 1 ul well solution. |
