4P2F
Monomeric form of a single mutant (F363R) of Mycobacterial Adenylyl cyclase Rv1625c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.890, 67.370, 101.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.580 - 2.050 |
| R-factor | 0.18176 |
| Rwork | 0.179 |
| R-free | 0.23331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ET6. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.728 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.580 | 2.103 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.076 | 0.452 |
| Number of reflections | 12712 | |
| <I/σ(I)> | 19.8 | 4.3 |
| Completeness [%] | 99.0 | 97.65 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 0.15M Ammonium sulphate, 0.1M Bis-Tris pH 5.5, 30% PEG 10K |
