4P1Y

Crystal structure of staphylococcal gamma-hemolysin prepore

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	197.056, 197.050, 192.965
Unit cell angles	90.00, 99.04, 90.00

Refinement procedure

Resolution	44.399 - 2.992
R-factor	0.2312
Rwork	0.230
R-free	0.25430
RMSD bond length	0.008
RMSD bond angle	1.037
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	3.099
High resolution limit [Å]	2.992	2.992
Rmerge	0.186	0.622
Number of reflections	139696
<I/σ(I)>	4.73	1.53
Completeness [%]	95.5	77.76
Redundancy	3.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	298	60% MPD, 0.1M sodium acetate (pH 4.8), 0.15M di-sodium hydrogen phosphate