4P18
Crystal Structure of frog M ferritin mutant D80K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.930, 238.430, 119.690 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 75.350 - 1.910 |
| R-factor | 0.19791 |
| Rwork | 0.196 |
| R-free | 0.23082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lqh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.253 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.380 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.149 |
| Number of reflections | 508671 | |
| <I/σ(I)> | 9.4 | 5.3 |
| Completeness [%] | 97.2 | 91.9 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 1.0 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.6 |
