4P0S

human Mus81-Eme1-3'flap DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	103
Detector technology	CCD
Collection date	2013-09-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	92.442, 250.758, 430.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.978 - 6.000
R-factor	0.2533
Rwork	0.250
R-free	0.30790
RMSD bond length	0.018
RMSD bond angle	2.353
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	5.920
Number of reflections	16874
<I/σ(I)>	43.7
Completeness [%]	99.5
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG400, Tris, DTT