4P0A

Crystal structure of HOIP PUB domain in complex with p97 PIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	80
Detector technology	CCD
Collection date	2013-04-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	48.040, 48.040, 266.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.604 - 2.300
R-factor	0.2018
Rwork	0.199
R-free	0.24870
RMSD bond length	0.010
RMSD bond angle	1.192
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	53.320
High resolution limit [Å]	2.300
Number of reflections	15446
<I/σ(I)>	14.9
Completeness [%]	99.9
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 6.5