4OYN
Fifteen minutes iron loaded human H ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.910, 183.910, 183.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.090 - 1.430 |
| R-factor | 0.16757 |
| Rwork | 0.167 |
| R-free | 0.17948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.981 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.510 | 1.510 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.068 | 0.417 |
| Number of reflections | 49578 | |
| <I/σ(I)> | 27.9 | 7.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.6 M MgCl2 and 0.1 M bicine pH 9.0 |
