4OW1
Crystal Structure of Resuscitation Promoting Factor C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.230, 89.930, 78.090 |
| Unit cell angles | 90.00, 115.08, 90.00 |
Refinement procedure
| Resolution | 38.420 - 1.900 |
| R-factor | 0.20653 |
| Rwork | 0.205 |
| R-free | 0.23548 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.823 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.970 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.382 |
| Number of reflections | 64089 | |
| <I/σ(I)> | 8.3 | 2.6 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1 M NaCitrate pH 5, 22% (w/v) PEG 6000 |
