4OV2
Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.680, 88.800, 100.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.594 - 2.600 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.30820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YOU |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.292 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.594 | 2.770 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 20185 | |
| <I/σ(I)> | 2.3 | 1.1 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 0.2 M sodium chloride, 0.1 M MES, pH 6.0, 16% w/v PEG6000, VAPOR DIFFUSION, SITTING DROP |
