4OTP

Crystal structure of the catalytic domain of the human RioK1 atypical protein kinase in complex with ADP/Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	78.760, 78.760, 110.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.949 - 2.700
R-factor	0.2077
Rwork	0.204
R-free	0.24340
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.950	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.066	0.215
Number of reflections	11336
<I/σ(I)>	12.69	3.4
Completeness [%]	100.0	100
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	28% PEG400, 0.1 M HEPES, pH 7.5, 0.2 M calcium chloride, VAPOR DIFFUSION, temperature 293K