4OSU
Crystal structure of HCMV gB-neutralizing SM5-1 Fab fragment
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.645, 92.645, 124.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 1.870 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.047 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 43280 | |
| Completeness [%] | 99.9 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
