4ORK
Crystal Structure of the Phosphotransferase Domain of the Bifunctional Aminoglycoside Resistance Enzyme AAC(6')-Ie-APH(2'')-Ia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.685, 95.697, 91.149 |
Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
Resolution | 36.200 - 2.300 |
R-factor | 0.1882 |
Rwork | 0.185 |
R-free | 0.24490 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.200 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.038 | 0.510 |
Number of reflections | 62509 | |
<I/σ(I)> | 13.6 | 1.6 |
Completeness [%] | 93.3 | 96.6 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M MgCl2, 0.1 M Hepes, pH 7.5, 30% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |