4OR5
Crystal structure of HIV-1 Tat complexed with human P-TEFb and AFF4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.862, 186.739, 108.661 |
| Unit cell angles | 90.00, 120.24, 90.00 |
Refinement procedure
| Resolution | 41.900 - 2.900 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.083 | 0.453 |
| Number of reflections | 67551 | |
| <I/σ(I)> | 18.1 | 2.2 |
| Completeness [%] | 95.0 | 87.1 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 50 mM MES pH6.5, 3.7-3.75% w/v PEG 20000, 5 mM YCl3, 200 mM NDSB 211, 2 mM TCEP pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
