4OOR
Ancestral Steroid Receptor 2 DNA binding domain in complex with a steroid response element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.472, 80.374, 115.645 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 30.583 - 2.701 |
| R-factor | 0.1926 |
| Rwork | 0.191 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1593) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 6.650 | 2.700 |
| Rmerge | 0.082 | 0.043 | 0.356 |
| Number of reflections | 23370 | ||
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 97.9 | 99.1 | 82.3 |
| Redundancy | 3.5 | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 20% PEG3350, 50 mM ammonium acetate, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
