4OO1

Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 21 21 2
Unit cell lengths	193.292, 200.083, 97.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.426 - 3.300
R-factor	0.2288
Rwork	0.227
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4IFD 2NN6 AND 2HBL
RMSD bond length	0.002
RMSD bond angle	0.474
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.061	0.653
Number of reflections	56332
<I/σ(I)>	15.5	1.4
Completeness [%]	97.7	96.9
Redundancy	5.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291	7-11% PEG3350, 100 mM MES pH 6.7, 4-15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 291K