4ONL

Crystal structure of human Mms2/Ubc13_D81N, R85S, A122V, N123P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	105
Detector technology	CCD
Collection date	2013-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03316
Spacegroup name	P 21 21 21
Unit cell lengths	43.933, 74.942, 92.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.059 - 1.350
R-factor	0.1727
Rwork	0.171
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.396
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.370
High resolution limit [Å]	1.350	3.660	1.350
Rmerge	0.034	0.018	0.607
Number of reflections	66851
<I/σ(I)>	19.7
Completeness [%]	98.9	91.4	97.2
Redundancy	3.7	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K