4ONI
Structure of Human Orphan Receptor LRH1 bound to two bacterial phospholipids
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.867, 59.856, 73.940 |
| Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.800 |
| R-factor | 0.1581 |
| Rwork | 0.156 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yuc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.855 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.772 | 46.481 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.039 | 0.797 | |
| Rmeas | 0.042 | 0.892 | |
| Rpim | 0.015 | 0.393 | |
| Total number of observations | 12545 | 27575 | |
| Number of reflections | 49141 | ||
| <I/σ(I)> | 13.2 | 30.8 | 2.4 |
| Completeness [%] | 94.8 | 99.8 | 72.4 |
| Redundancy | 7 | 7.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 20-30% PEG 3350, Bis Tris, 5% glycerol, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
