4OML
Crystal structure of the intertwined dimer of the c-Src tyrosine kinase SH3 domain mutant Q128R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-03 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 46.546, 46.546, 128.034 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.220 - 1.600 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jz4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.220 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.073 | 0.654 |
Number of reflections | 11086 | |
<I/σ(I)> | 21.4 | 4.4 |
Completeness [%] | 97.0 | 93.9 |
Redundancy | 12.9 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 298 | 0.1M sodium acetate, 1.6 M ammonium sulphate, 10%PEG 300, 10%Glicerol, pH 5.0, vapor diffusion, hanging drop, temperature 298K |