4OM8
Crystal structure of 5-formly-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) 5-dehydrogenase, an NAD+ dependent dismutase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-14 |
| Detector | Saturn A200 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.805, 44.212, 109.343 |
| Unit cell angles | 90.00, 116.35, 90.00 |
Refinement procedure
| Resolution | 27.336 - 1.550 |
| R-factor | 0.1567 |
| Rwork | 0.155 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fim |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.534 |
| High resolution limit [Å] | 1.500 | 1.495 |
| Number of reflections | 75976 | |
| Completeness [%] | 96.5 | 87.43 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.2M NH4-acetate, 0.1M Na-acetate pH 5.0, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
