Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.207, 106.747, 68.341 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 39.045 - 2.899 |
| R-factor | 0.2118 |
| Rwork | 0.210 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.347 | 3.050 |
| High resolution limit [Å] | 2.893 | 2.893 |
| Rmerge | 0.058 | 0.286 |
| Number of reflections | 19005 | |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 97.9 | |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 16% PEG3350, 12% isopropanol, saturated L-tryptophan, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
