4OJF
Humanised 3D6 Fab complexed to amyloid beta 1-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 60.162, 83.430, 88.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.704 - 1.998 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hix |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.070 |
| High resolution limit [Å] | 1.998 | 4.310 | 2.000 |
| Rmerge | 0.104 | 0.051 | 0.369 |
| Number of reflections | 30773 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 14.4 | 13.7 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 25%(w/v) PEG 6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
