Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2013-11-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.03 |
Spacegroup name | P 61 |
Unit cell lengths | 78.560, 78.560, 224.801 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.329 - 3.101 |
R-factor | 0.2288 |
Rwork | 0.225 |
R-free | 0.26670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.914 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1448)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 13924 | |
Completeness [%] | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1 M HEPES, pH 7.4, 5% PEG6000, 5% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |