4OGQ

Internal Lipid Architecture of the Hetero-Oligomeric Cytochrome b6f Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97856
Spacegroup name	P 61 2 2
Unit cell lengths	159.228, 159.228, 365.879
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.570 - 2.501
R-factor	0.2023
Rwork	0.201
R-free	0.23240
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.766
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.570
High resolution limit [Å]	2.500
Number of reflections	498334
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG550MME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG550MME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K