4OF5
Refinement of RAGE-DNA complex in 3S59 without DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 61 |
| Unit cell lengths | 79.121, 79.121, 224.045 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.560 - 2.803 |
| R-factor | 0.2208 |
| Rwork | 0.220 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3S59 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.627 |
| Refinement software | PHENIX (1.8.3_1479) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 10% PEG6000, 0.1 M Tris_HCl pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
